Abstract
In the leaf vein development, local distribution of auxin, which is dynamically changed during the leaf development, is known to be prerequisite for leaf-vascular formation. It is known that polar transport of auxin is necessary for the proper distribution of auxin and that polar localization of PIN proteins is required for the polar auxin transport. However, molecular mechanisms for regulated auxin distribution are still largely unknown. Toward the understanding the mechanisms of formation of leaf-venation pattern, we have identified NO VEIN (NOV) gene which is necessary for prepro/procambial cell formation in leaves. It turned out that NOV is required for formation of developmentally regulated auxin-distribution pattern both in the leaf and in the embryo. NOV encodes a protein with unknown function. Analyses of expression and subcellular localization of NOV, genetic interaction between NOV and GNOM, expression and subcellular localization of PIN proteins in nov mutants will be presented.