Abstract
The primitive red alga, Cyanidioschyzon, has only a set of organelles, such as nucleus, mitochondrion and chloroplast in the cell. The cell cycle of this organism coordinates with division cycle of chloroplast and mitochondrion. However, mechanisms for the regulation of this coordinated cell cycle have not been elucidated. Here, using synchronized cultivation technique, we investigated regulatory mechanisms for organelle DNA replication and that to coordinate organelle cell cycle with that of the nucleus.
We identified that replication of the chloroplast genome and mitochondrial genome starts before that of the nuclear genome by real-time PCR method and BrdU incorporation. We also identified that any inhibitor effect. These results suggest that proliferation cycle of each organelle in Cyanidioschyzon is regulated independently and that unknown mechanisms may involve to coordinate organelle DNA replication and division in Cyanidioschyzon.
