Abstract
It has been established that a green alga, Chlamydomonas reinhardtii, induces a carbon-concentrating mechanism (CCM) and accumulates inorganic carbon (Ci) under CO2-limiting conditions. However, CO2 transporters remain to be identified. We previously demonstrated that a CCM regulatory mutant lcr1, which is deficient in a Myb transcription factor, LCR1, was unable to induce three low-CO2 inducible genes, Lci1, Lci6, and Cah1, and showed a decreased affinity against Ci.1) Since Lci1 encodes a membrane protein, we introduced a chimeric Lci1 gene, which was fused with an upstream region of Nia1 gene, into the lcr1 mutant to induce the expression of Lci1 by switching nitrogen source in the media from ammonium to nitrate in high-CO2 condition. We will discuss the function of LCI1 based on the CO2-uptake activity and affinity against inorganic carbon of the transformants carrying chimeric Lci1.
1) Yoshioka et al. Plant Cell (2004)
