Abstract
Nitric oxide has been thought to play a key role on signal transduction involved in many important physiologic functions. To elucidate the molecular mechanisms for NO signaling in higher plants, we made two-types of the transgenic Arabidopsis excessively producing NO. One (cdeiNOS) constitutively overexpressed NOS gene derived from Deinococcus radiodurans, and the other (ideiNOS) transiently overexpressed deiNOS gene with the ethanol-inducible promoter derived from Aspergillus nidulans. We previously showed that the expression levels of many genes already reported as NO response genes in some articles are almost same as that of WT using microarray analysis in cdeiNOS. To analyze NO early response genes, we analyzed the expression pattern of genes in ideiNOS using microarray analysis. We observed remarkable differences in the expression levels of 1731 genes (up-regulated 887,down-regulated 844) between no-treatment and ethanol-induced ideiNOS. We are analyzing genes which exhibited similar expression profiles between cdeiNOS and ideiNOS.
