Abstract
In higher plants, alcoholic fermentation is important to survive under hypoxic conditions. Alcohol dehydrogenase (ADH), which is responsible for alcoholic fermentation, is required to supply NAD+ to glycolytic pathway. We characterized the reduced adh activity (rad) mutant, whose coleoptile elongation is repressed under submergence. It was found that the reduction of ADH activity was caused by decrease in the amount of ADH1 protein as a result of point mutation in the Adh1 gene.
In this study, we analyzed difference in gene expression between the rad mutant and the wild type during coleoptile elongation. Thus, the coleoptiles were isolated by use of Laser Microdissection (LM). Thereafter, the rice 22K oligo-DNA microarray was performed using RNA extracted from the LM-isolated tissues. We selected 315 up-regulated genes and 153 down-regulated genes in the rad mutant (p-value<0.05). Now, functional analyses of some interesting genes are under way.
