Abstract
The cell line GTH4 was generated from the F1 hybrid of Nicotiana gossei x N. tabacum which reveals lethality at seedling stage. The cells proliferated at 37 C but died at 26 C. Inhibitor treatments suggested that cell death depends on expression of genes in the cell at 37 C. The mutant cell line GTH4S does not die, suggesting that GTH4S does not express the genes which are involved in the cell death. Accordingly, it was worth comparing the differences in the genes expressed between GTH4 and GTH4S. To find out the genes involved in the cell death process, 100 x 103 cDNA clones of GTH4 have been analyzed by differential screening using cDNA probes prepared by RNA of GTH4 or GTH4S. Three clones which were specific to GTH4, NADH dehydrogenase subunit 1 of N.tabacum and two genomic sequences of chloroplast of Solanaceae plants, were found.
