Analysis of Knockout Mutants for Gibberellin Receptor AtGID1s in Arabidopsis thaliana.

Abstract

Three receptor genes (AtGID1a, AtGID1b, and AtGID1c) for a phytohormone gibberellin (GA) exist in Arabidopsis genome. The AtGID1s proteins are able to bind with GA, and the over-expression of each AtGID1s gene could complement dwarf phenotype of rice GA-receptor mutant gid1-1. In this study, we elucidate the function of AtGID1s using T-DNA and Ds insertion mutants obtained from RIKEN GSC and ABRC. We could not detect obvious phenotypic change in the homozygous single mutants (atgid1a, atgid1b, atgid1c) suggesting that the function of AtGID1s is highly redundant. Thus we established double mutants (atgid1ab, atgid1ac, and atgid1bc) to detect any functional difference. The phenotypes of double mutants including germination, stem elongation, flowering and seed development are examined. The result of crossbreeding experiment to establish triple mutant (atgidabc) will be also reported.