Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 48
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Host: The Japanese Society of Plant Physiologists

Involvement of clathrin in stabilizing the mitotic spindle
*Hiroshi Tahara, Etsuo Yokota, Hisako Igarashi, Hidefumi Orii, Toshio Sano, Seiichiro Hasezawa, Seiji Sonobe, Yoshinobu Mineyuki, Teruo Shimmen
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Abstract
We have previously found that two types of clathrin signals, dot and diffuse, located in mitotic spindle in BY-2 cells stained with an antibody against clathrin heavy chain. In the present study, we analyzed the live image of BY-2 cells expressing YFP-clathrin light chain. Dot signals were observed around spindles. In addition, diffuse signals were also observed on spindle. Analyses were carried out in BY-2 cells expressing both C-terminal region of clathrin heavy chain and GFP-tubulin. The metaphase was significantly prolonged and the spindle became aberrant during the anaphase. These results suggested that clathrin is involved in stabilizing the mitotic spindle.
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© 2007 by The Japanese Society of Plant Physiologists
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