INCREASED LEVEL OF POLYPLOIDY2 Controls the Endocycle in Arabidopsis thaliana

Abstract

Endoredulication is one of cell cycle that increases ploidy without mitosis. The molecular mechanisms that controls endoreduplication are poorly understood. To reveal this, we isolated several dominant mutants that increase polyploidy from Arabidopsis activation tagging lines using flow-cytometer. increased level of poliploidy2-D (ilp2-D) was one of the mutants that showed increased polyploidy in both light-grown and dark-grown seedlings compared with the wild-type. To examine ILP2 function, we isolated two T-DNA insertional mutants. The both mutants reduced ploidy level in light-grown 20-day-old first leaf cells. We observed in vivo localization with ILP2 protein fused to GFP. That revealed that ILP2 protein localized in chloroplasts.