Abstract
Endoreduplication is a special cell cycle defined as the replication of DNA without cell division.
In plant, this phenomenon is often observed in various tissues, and it controls organ size and development.
Because the molecular mechanism of endoreduplication is poorly understood,
we screened novel endoreduplication mutants to make clear this mechanism in Arabidopsis.
In previous study, 17 mutants that increased of DNA content have been isolated from Activation Tagging lines. But the Activation Tagging lines have the problem that is difficult to find the corresponding gene.
Therefore, we tried new screening that used the Fox lines, which is over expression Arabidopsis full length cDNA transformants, and it is easy to find the gene that cause increasing of ploidy.
We screened 1,215 Fox lines by a Flowcytometer, resulted in 23 mutant candidates showing increased polyploidy.
Here we report the screening and the phenotypes of mutants.
