Abstract
We isolated high-light and heat-shock stress-inducible genes, including heat shock transcription factor (HsfA2) from Arabidopsis. The transcript level of HsfA2 increased under several types of stressful condition. We found that HsfA2 regulates transcription of a large number of HSPs and the defense-related genes (Plant J, 2006). Here we investigated in detail the gene regulation system via the HsfA2 under the high-light and oxidative stress conditions. The transcript levels of HsfA2 target genes as well as HsfA2 were induced under high-light irradiation or H2O2 treatment. In knockout-HsfA2 plants, the induction of HsfA2 target genes was reduced under high-light irradiation. Dual luciferase assay showed that only one HSE is sufficient to induce the expression of HSP18.1-CI, a HsfA2 target gene. Now, we are trying to investigate the regulation system of the other HsfA2 target genes via the HsfA2.
