Abstract
Plants with 'Purple leaf' that anthocyanin appears constitutively in the leaf are very popular and has been used in the arranged cut flowers and the gardening plants. 'Purple leaf' plant has been obtained as a bud mutation from various plants. But it is not known in the petunia that is one of the most often-used bedding plants. The OSB2 gene has been cloned from the Purple leaf locus of rice and codes the transcription factor of the bHLH type. When having introduced the OSB2 gene with the CaMV 35S promoter into petunia var. 'Purple Wave', anthocyanin accumulated only in the leaf edge part of the petunia. When the transgenic petunia crossed with the other petunias transformed with the gene of another transcription factor of the myb type, some of the offspring accumulated a lot of anthocyanin in the leaf and the flower. These results will be discussed.
