Abstract
In order to elucidate the root elongation mechanism under drought stress, we are studying regulatory mechanism of the expression cell wall-related genes that express in Arabidopsis root tip. Bioluminescence from transgenic Arabidopsis root tips were observed, when promoter of CesA 1, 3, 6, AtEXP11, XTH5, 14, AtFUT6 were fused with luciferase reporter gene. Bioluminescence of the XTH14-LUC reporter gene was reduced under osmotic stress, which inhibits root elongation. Therefore, the XTH14-LUC reporter gene is suitable to elucidate regulatory mechanism of the expression of cell wall-related gene under abiotic stress.
Promoter analysis of XTH14 has revealed that the region between -137 and -205 from the transcription start site is important for the expression in root tip. A deletion of the region between -63 to -137 also reduced the reporter activity. These regions include W-box, G-box. We discuss roles of these cis-elements on the expression of XTH14.
