Abstract
Plants possess several classes of photoreceptors to perceive lights and regulate the development throughout their life cycle. One of these photoreceptors is a phytochrome (phy) family, which is important for a wide range of developmental responses. In this study, we aimed to investigate the differences in metabolic profiles during de-etiolation between wild-type Arabidopsis thaliana and phyA mutant by means of GC-TOF/MS analysis. Etiolated WT and phyA seedlings were irradiated with continuous far-red light (FRc), or white light. Primary metabolites of the seedlings were profiled by GC-TOF/MS to find phyA-regulated metabolic pathways. Comparison of metabolite levels of the seedling under FRc revealed several metabolites that contribute to the difference between WT and phyA seedlings. Several amino acids and major sugars were found in higher amounts in phyA than in WT seedlings under FRc. There were also significant differences in metabolite levels between WT and phyA seedlings during de-etiolating under white light.
