Abstract
Brassica napus leaf protoplasts become swollen and finally degrade during culture. The process through which the death occurred were subjected to apoptotic-like manner. Glutamate dehydrogenase (GDH) catalyzes the amination of 2-oxoglutarate and the deamination of L-glutamate. GDH of B. napus consists with a and b subunits that are encoded by two genes (GDH1, 2), respectively and forms seven isoforms (GDH1~7).
de novo synthesis of a subunits was post-translationally regulated in B. napus leaf protoplasts. While, activation of a subunits in wounded tissue were regulated by reduction of disulphide bond. Leaf protoplasts were subjected to oxidative stresses and in different redox state, which led to prevent translational inhibition. Leaves treated with SA and JA in an acidic conditions showed the same GDH pattern as protoplasts.
These results suggest that a subunits of GDH are stress responsive and could be involved in redox regulation and pH stability.
