Abstract
Exposure of rice plants at booting stage to moderate low temperature results in dramatic decrease in fertility. The phenomenon is associated with male sterility, due to the disfunction of tapatum cells. To address molecular events during this process, we carried out transcriptome analysis using a 22K chip (Agilent).
Since we observed notable difference in fertility in plants chilling (12°C) treated for 2d (70%) and 4d (25%). RNAs were prepared from anthers of 2d and 4d plants and plants with 1 day recovery at normal temperature (2d+1 and 4d+1), and were subjected to microarray analysis. Analysis of gene expression profiles revealed no significant change in major metabolic pathways, whereas genes involved in photo-system I, II are dramatically diminished in 4d+1 plants, indicating that there is a correlation between disorder of photo-system and diminished fertility by 4d chilling-treatment.
