Abstract
When incinerated ashes contact with water, leaching of chemical toxic factors such as high alkaline pH, salts and heavy metals may occur. We have assessed the safety of incinerated ash-leachates by giving them to rice plants. Consequently, high concentration of Cu was one of the toxic factors in ash-leachates. Thus we examined the effects of excess Cu to rice plant. Rice roots were treated with the hydroponic solutions containing 10 μM, 45 μM or 130 μM of CuCl2 individually. After 1 day of the treatments, both photosynthesis and transpiration of leaves declined with increasing Cu concentration in the hydroponic solution. From the results of DNA microarray analysis, genes related to defense, lipid metabolism or stress response were up-regulated, and those related to photosynthesis or transport were down-regulated by Cu-doses. For the further discussion, accumulation of chemical elements in rice plants will examine. (Supported by the leading project of the MEXT.)
