Functional Genomics of Stress-Responsive Transcription Factors by Fox Hunting System

Abstract

The Fox Hunting System (Full-length cDNA over-expresser gene-hunting system), a system for random over-expression of a normalized Arabidopsis full-length cDNA library, was applied for the functional analysis of stress-inducible transcription factors. An agro-library containing forty three stress-inducible transcription factors was constructed and used for transformation of Arabidopsis plants. Several salt-tolerant lines expressing F39 gene were screened from the T1 transgenic plants. T2 lines expressing F39 gene also showed obvious salt tolerance. Moreover, based on the microarray analysis and the analysis of loss-of-function mutants, an important role of F39 in salt stress responce will be discussed.