Abstract
We previously showed that the promoter of the Ri-plasmid-borne agropine synthase gene (Ri-ags) of Agrobacterium rhizogenes had tissue-specificity to root and wound-responsiveness in leaf and stem in tobacco. Because of very rapid emergence of mRNA after wounding and unnecessariness of de novo protein synthesis the wound response was thought to be a primary one. Because a signal transduction pathway for this wound response in tabacco was not elucidated, we planned to analyze it with Arabidopsis genetically. In this study, we introduced a fusion gene of the Ri-ags promoter and the GUS reporter into Arabidopsis, then investigated the expression of the fusion gene in T2 plants. As in a tabacco plant, a high GUS activity was observed in roots. But, no wound response was observed in leaves. Thus, in Arabidopsis the Ri-ags promoter is thought to be controlled differently than in tabacco.
