Current Status of Genome Sequencing Projects of Plants and Endophytic Bacteria at KDRI

Abstract

Tomato and eucalyptus represent key species with great potential for conducting genomic research. As a member of the International Tomato Sequencing Project, we launched on sequencing of the chromosome 8. Our mission is to sequence the euchromatin, the estimated size of which is 17Mb. As of Nov. 2006, we finished 40 BACs (4.4Mb), and 20 BACs are in sequencing pipeline. The genome size of eucalyptus is estimated to be 650Mb. The Whole Genome Shotgun sequencing strategy was applied to produce the draft sequence. To date, three million reads were produced. As the third project, we launched on the genome sequencing of twenty-one endophytic bacteria. Some of them have been reported to contribute to the nutrition and increase the disease resistance of host plants. Sequencing of threefold coverage of each genome was achieved by producing approximately 108Mb draft sequences in total. We will present the latest status of our projects.