Abstract
Plant metabolomics has various potential applications; for example improvement for the useful products. To achieve this purpose, we have been trying to develop methodologies to describe the metabolic network analysis of atomic redistribution process using uniformly stable isotope labeled plant cells measured by hetero-nuclear NMR.
Previously, we showed the atomic redistribution analysis of carbon source [13C6] glucose using simple closed system (Arabidopsis T87 cells). At this time, we will show detailed strategies of signal assignments by a combination of multi-dimensional NMR methods such as 1H-13C HSQC, HCCH-TOCSY, HCCH-COSY, HCACO. Introduction of these correlation spectra exhibits overcoming of signals overlapping and unambiguous signal assignments. By use of these assigned NMR signals, we monitored the time-dependent changes of the signal intensities at atomic level in order to follow the metabolic network at atomic level.
