Abstract
[Objective] Catharanthus roseus produces about 130 terpenoid indole alkaloids (TIAs), including valuable medical substances. To date, many genetic, molecular biological, and biochemical studies on TIAs biosynthetic pathway were conducted, however, precise and comprehensive analysis of whole metabolism in C. roseus cell has not been conducted yet. In this study, metabolic profiling of methyl jasmonate (MeJA)-treated C. roseus shoot and cultured cell was obtained in order to analyze metabolic alteration when TIAs biosynthesis is activated. [Method] Treatment of MeJA was performed by transferring shoot or suspension cultured cell to the medium added with MeJA. Time-course sampling was conducted and the metabolites were extracted and purified from these samples. Sugars, amino acids and organic acids were analyzed by GC/MS. Sugar phosphates, nucleotides and CoA compounds were analyzed by CE/MS. And TIAs were determined by UPLC/MS. Obtained metabolic profiles were applied to multivariate analysis.
