Abstract
The genes for building up chloroplasts express in leaves of plants by perception of light. To know the underlying mechanism, we the selected mutants ces102 and ces103 (callus expression of RBCS) of Arabidopsis by activation tagging on the criterion of expression of RBCS-3B in calli. The gene of At3g05490 was activated in ces102, and the same phenotype was reproduced by ectopic expression of this gene, leading to the conclusion that the CES102 is the At3g05490 product. Fluorescence of CES102-sGFP was observed in endoplasmic reticulum by transient expression. As for ces103, both At3g59860 and At3g59870 were activated and the greening phenotype was reproduced by over-expression of any of these genes. While expression of RBCS-3B in the At3g59860-expressing calli was almost compatible with that in ces103, the ectopic expression of At3g59870 resulted in elevation of RBCS-3B transcripts These results indicate that CES103 would be At3g59860.
