Abstract
Crops that were conferred useful traits by genetic engineering have potential to increase crop yields, improve agricultural practices, or add nutritional quality to products. Despite broadly expanding of genetically modified crops over the world, risk of undesirable diffusion of antibiotics resistant genes to ecosystem still exists. To avoid this problem, development of selection marker derived from native plant gene would be favorable for plant transformation. We are now intending to develop a novel technique, with the aid of self-cloning application. As the candidate genes for native selection marker, we focused plant transcription factors that response to abiotic stress. Because some of the defective mutants show tolerance to abiotic stress, we attempt to isolate transgenic Arabidopsis plants that shows tolerance to various stress, such as salt and drought, and for herbicide exposure by CRES-T system. Molecular approaches and tools for the development of self-cloning in Arabidopsis will be discussed.
