Abstract
Real-time monitoring of gene expression using a bioluminescence reporter strain is one of the most powerful methods for the analysis of gene expression in living cells with a high sensitivity, a broad dynamic range, and a high time-resolution. The method allows easy isolation of a large number of mutants affecting the expressions of any genes in the genome. However, there were many problems in previous systems. We have developed a high-throughput real-time bioluminescence monitoring system, which consists of two types of high-throughput bioluminescence monitoring apparatus and the bioluminescence-analyzing program RAP. The system enables the automatic monitoring and analysis of bioluminescence from 4,800 individual samples in one assay under uniform light conditions.
Here, we will introduce the real-time bioluminescence monitoring system and the cloning and analysis the circadian clock genes in cyanobacteria and Arabidopsis.
