Supplement to Plant and Cell Physiology Vol. 49
Conference information
Host: The Japanese Society of Plant Physiologists
Function of AOX under low temperature
Pages 0016
Details
Host: The Japanese Society of Plant Physiologists
Pages 0016
In the plant respiratory chain, the ATP-uncoupling cyanide-resistant pathway catalyzed by the alternative oxidase (AOX), is supposed to prevent ROS production and maintain a carbon/nitrogen (C/N) ratio.
The aim of this study is to clarify roles of AOX in low temperature. We used Arabidopsis thaliana WT and aox1a knock-out transgenic treated at 4C. In WT, AOX protein amounts and cyanide-resistant respiration rates increased in 4C, but total respiratory rates were higher in aox1a than those in WT. In aox1a, an ATP-uncoupling NADH dehydrogenase, NDB2, was significantly induced. A malondialdehyde amount, an index of oxidative damage, in aox1a was significantly lower than that in WT. These results suggested that a lack of AOX did not induce ROS production and damages, and was complemented by other proteins. However, the C/N ratio in aox1a was significantly higher than that of WT, which suggesting that AOX maintain the C/N ratio under low temperature.
