Abstract
Photosynthesis is one of the most important determinants of crop productivity. To identify photosynthesis-related genes of rice, we generated Rice-Arabidopsis FOX lines that rice full-length cDNAs were over-expressed in Arabidopsis. We have used imaging of chlorophyll fluorescence to screen photosynthesis-related mutants. We generated transformants overexpressing each cDNA isolated from candidates, resulting that thirteen re-transformed lines showed the same phenotype as the original lines. Candidates could be classified into five categories depending on the chlorophyll fluorescence parameters. We analyzed gene expression profile of three lines showing similar photosynthetic characteristics using DNA microarray. We found commonly regulated genes among three candidates, suggesting that candidates have shared influences on gene expression. We analyzed the metabolites of candidate lines, resulting that each category showed similar metabolite profile. This research is supported by the Special coordination funds for Promoting Science and Technology entitled Rapid identification of useful traits using rice full-length cDNAs.
