Establishment of High Sensitive Microanalytical Method for Lignan Profile and Light Condtion Correlation Analysis in Forsythia suspensa

Abstract

Lignans' various useful physiological effects on humans have been attracting attention. For example, lignans have antioxidant and anti-cancer effects. However, it was quite difficult to establish a comprehensive analytical system for lignans as various analogs and metabolic intermediates exist in plants; some in extremelyt small amount. In this study, we established a high-sensitive microanalytical analytical method for lignans and phenylpropanoids using capillary LC-ESI-MS. Multiple reaction monitoring (MRM) was adopted for the analysis. Limit of detections were several fmol. Using this analytical method, we investigated correlations between light conditions that are a critical factor for plant metabolism, and lignans profile in leaves of a lignan-rich plants, Forsythia suspensa.
As a result, ten lignan related compounds were detected in leaves of F. suspensa. Additionally, the amount of five lignans was increased by coutinuous blue light irradiation.