Host: The Japanese Society of Plant Physiologists
Pages 0041
Coumarins consist of 2H-1-benzopyran-2-one core structure and are ubiquitously found in the plant kingdom. They are originated from the general phenylpropanoid pathway via ortho-hydroxylation of cinnamates, trans-cis isomerization of the side chain and lactonization. We have already reported that the feruloyl-CoA 6'-hydroxylase of Arabidopsis thaliana (AtF6'H1) is involved in ortho-hydroxylation of ferulate unit, which is the key step of scopoletin biosynthesis. There is less information about the steps in the lactone formation of coumarins following AtF6'H1 catalysis. In this study, we analyzed formation of scopoletin after AtF6'H1 reaction. We found that formation of scopoletin non-enzymatically proceeds in the mixture.