Abstract
In Arabidopsis thaliana, CURLY LEAF (CLF) and SWINGER (SWN) are known as Polycomb complex Group (PcG) factor that play key roles by modulating histone methylation. It is suggested that PcG regulate repression of embryonic traits by the fact that clf swn forms embryo-like structure (ELS) after germination, but the molecular mechanisms are still unclear.
We had already reported that the formation of ELS on shoot apical meristem of the clf swn was suppressed by cultivation at low temperature (10ºC). To identify developmental stage for repressing embryonic traits, the clf swn was transferred from 21ºC to 10ºC at various time after sowing. On the other hands, ELS were formed when the clf swn was transferred after the stage of cotyledon expansion, CLS formation was induced as the higher concentration of 2,4-D. These results suggest that auxin should be involved in ELS formation in clf swn at the stage of cotyledon expansion.
