Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 49
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Promoter Analysis of OsChia4a That is Regulated by RERJ1, a Jasmonic acid-responsive Transcription Factor in Rice
*Kouji MiyamotoKazunori OkadaTetsuya ChujoTakashi SuzumuraTakashi OtaniKyoko KiribuchiYoshiaki NagamuraNaoto ShibuyaHideaki NojiriHisakazu Yamane
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Pages 0072

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Abstract
Jasmonic acid (JA) is a plant hormone that acts as a secondary signaling molecule in plant defense responses. RERJ1 that encodes a bHLH transcription factor was isolated as a JA-responsive gene from rice suspension cells. Previously, we reported that RERJ1 functions as a transcriptional activator and the expression of OsChia4a, which encodes class 4 chitinase, was induced in a RERJ1-dependent manner.
Here, we identified a JA-responsive cis element in the promoter region of OsChia4a by reporter-gene assay. The deletion analysis showed that the region from -515 to -265 bp in the promoter contains JA-responsiveness, and we found that the region contains three E-boxes. Subsequent mutation analysis of three E-boxes indicated that an E-box at -448 to -443 bp is essential for responding to JA and also for RERJ1-dependent expression of OsChia4a. Now, we are analyzing the binding of RERJ1 to this E-box by chromatin immunoprecipitation.
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© 2008 by The Japanese Society of Plant Physiologists
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