Abstract
UVB can damage DNA by causing formation of cyclobutane pyrimidine dimer (CPD). CPD photolyase, which mediates CPD repair path, is one of crucial factor for determing UVB sensitivity in rice. Although plant cell has different genome in nuclei, chloroplast and mitochondria, it is unclear whether CPD photolyase functions in each organelle. We previously reported that UVB-induced CPD levels on each organelle genome decreased by exposure of the blue irradiation in rice leaves. These results suggest that CPD photolyase transits and functions in nuclei, chloroplast and mitochondria.
In order to clarify the localization of CPD photolyase in plant cell, in this study, we performed a western blot analysis using anti-rice CPD photolyase antiserum, and analysis of the cellular targeting of CPD photolyase-GFP fusion protein. As a result, CPD photolyase bands were detected in crude extract prepared from each purified organelle. Furthermore, GFP fluorescence was detected in nuclei and mitochondria.
