Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 49
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Acceptor limitation of electron transport in photosystem 1 in Arabidopsis
*Michito TsuyamaEiji GotohYoshichika Kobayashi
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Pages 0115

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Abstract

Chlorophyll fluorescence and absorbance changes at 830 nm were measured at low light in leaves of Arabidopsis thaliana. The level of 830-nm absorption (A830) was almost the same as that in darkness, indicating that P700 was in non-oxidized form. However, by illuminating a leaf with a pulse of strong light, A830 did not reach its maximum level. This indicates that there was a population of PS1 centers in which P700 remained non-oxidized and inactive. To investigate this reason, P700 oxidation by the pulse of saturation light was tested under various conditions. Consequently, it was found that reduced inactive P700 was not detected either in the presence of methyl viologen or under far-red light. These results indicate that a lack of electron acceptors in the stroma is responsible for the accumulation of reduced inactive P700 at low light.

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© 2008 by The Japanese Society of Plant Physiologists
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