Host: The Japanese Society of Plant Physiologists
Pages 0126
The phytohormone abscisic acid (ABA) plays an important role for seed dormancy. To explore genome-wide expression patterns and new components involved in ABA signaling in Arabidopsis seeds, comprehensive expression analysis was performed using whole-genome tiling array. In dry seeds, there was no difference in global gene expression between ABA-deficient mutant (aba2) and ABA-over-accumulated mutant (cyp707a1 cyp707a2 cyp707a3 triple mutant). At 24h after imbibition, the transcript levels of many genes in both mutants were drastically changed compared to those of wild type. Transcriptome analysis identified 380 ABA-upregulated and 540 ABA-downregulated AGI code genes at 24 h after imbibition. Moreover, whole-genome transcriptome analysis identified 4,884 non-redundant new transcription units (TUs) in Arabidopsis seeds. Among new TUs, 4,559 TUs probably encode hypothetical non-coding RNAs (ncRNAs). At present, we're proceeding analysis of the functional ncRNAs.