Host: The Japanese Society of Plant Physiologists
Pages 0130
Light is essential for plant growth. A number of studies have revealed both immediate activation of several signaling pathways and long-term responses of some metabolic pathways. To investigate light responses comprehensively, we performed the proteome analysis using thirteen-day-old dark-grown rice seedlings that were illuminated for 24 hr or not illuminated. Proteins from aerial parts were digested into peptides with trypsin, and the peptides obtained were then analyzed by the use of nano-flow liquid chromatography linked to a mass spectrometer. We identified 868 and 1026 proteins with non-illuminated and illuminated samples, respectively. As our data also indicated that relative amounts of respective proteins could be evaluated by the peak intensities, we performed comparative analysis of identified proteins. We are currently analyzing light-inducible proteins in more detail. We will discuss light-dependent systematic activation of respective metabolic and signaling pathways.
