Metabolic screening of Rice-Arabidopsis FOX (Full-length cDNA OvereXpressor) lines

Abstract

Metabolomics is a powerful high-throughput tool in the functional annotation of genes. We applied gas chromatography-time-of-flight mass spectrometry (GC-TOF/MS)-based techniques to detect changes in the metabolite profile of 345 3-weeks-old independent Arabidopsis lines overexpressing full-length rice cDNAs (Rice-Arabidopsis FOX lines).
Out of these 345 T2-lines analyzed, 234 showed a wild type phenotype and 111 exhibited an altered phenotype under defined growth conditions. The secondary screen of a total of 50 lines confirmed the metabolite change in 26 lines.
Data for one retransformed Arabidopsis line with a metabolic change in accordance with the results obtained in the primary and secondary screen will be presented. The line harbors a rice cDNA of unknown function. The linkage between metabolomic and transcriptomic changes in this line sheds light on the relationship between nitrogen-assimilatory and developmental pathways. The analysis of the corresponding rice overexpressor line will be presented as well.