Abstract
The environmental problems caused by CO2 emission are getting grave and the efficient application of plant oil is closely watched for one of the solution of those problems.
We screened genes that could increase the seed oil content by introducing chimera repressor transcription factor (TF)-fused genes based on CRES-T method into Arabidopsis thaliana. We had estimated that CRES-T method, known to be effective as creating dominant factors suppressing the expression of specific target genes with resultant dominant loss-of-function phenotypes, could improve the phenotype of oil content that is a quantitative trait.
We nondestructively measured the oil contents of recombinant T2 seeds into which TF-fused genes with 35S promoter were introduced by 1H pulse NMR. For the 1H pulse NMR, at least 3 mg seed was sufficient for quantification. As a result, we could get seeds containing more oil than that of the host plant.
