QTL Analysis of Amino Acid Contents in Rice Seeds.

Abstract

The quantitative trait loci (QTL) for the control of amino acid contents in rice (Oryza sative cv) grain were analyzed in order to investigate the genetic factor responsible for regulating primary metabolite levels in albumen tissues. Following the extraction of metabolites from the albumen of 85 back-crossed inbred lines (BILs) of rice derived from Sasanishi (japonica) and Habataki (indica), the concentration of 18 amino acids were determined by using LC-ESI-MS. The QTL analysis by the interval mapping method identified 25 QTLs for controlling the amino acid contents.