Host: The Japanese Society of Plant Physiologists
Pages 0181
We are interested in the underlying mechanisms how gain-of-function mutant rotundifolia4-1D has shorter leaves. In order to get some insights about it, we are studying ROT4 molecular function. ROT4 encodes small protein of 6.2kDa. Previously, we roughly showed that functional domain resides in conserved C terminal region of the molecule, but the precise region of it has not been characterized. Furthermore, whether this domain is processed out of the other part of the molecule has remained unknown. In the present study, we first identified the domain more precisely by overexpressing deletion series of the coding region. Secondly, we constructed two types of fusion protein (ROT4:GFP and GFP:ROT4) and analyzed the phenotype of the overexpressors. Subsequently, we detected the protein by immunoblotting to clarify if processing event occurs physiologically and if GFP-fusion protein is functional. Based on these and other experiments, we will discuss molecular function of ROT4.
