Host: The Japanese Society of Plant Physiologists
Pages 0183
Leaf develops along proximal-distal, medial-lateral and adaxial-abaxial axes. Although leaf is composed of leaf blade and petiole, the organogenesis along proximal-distal axis have received less attention. To understand mechanisms of junction formation between leaf blade and petiole, we carried out developmental analysis using wild-type Arabidopsis thaliana as well as a CYCB1;2:GUS transgenic line, which visualizes mitotic cells. As a result, it was found that leaf blade/petiole junction began to be visible at a relatively early stage of development, where mitotic cells distributed uniformly. Notably, although mitotic cells were known to distribute basipetally in developing leaf blade, this trend was reversed in the developing petiole. Similar analyses are underway using 35S:LEP and bop1bop2 that exhibit abnormal organogenesis along proximal-distal axis. Furthermore, to identify genes involved in the junction formation, we searched for enhancer trap lines specific to leaf blade/petiole junction.
