Mechanism of plant hypersensitive cell death induced by OsNAC4

Abstract

Plant hypersensitive cell death (HR cell death) is a form of plant programmed cell death and plays a major role in plant immunity. We previously demonstrated that OsNAC4, a plant specific transcription factor, is involved in induction of HR cell death in rice. To elucidate mechanism of HR cell death induced by OsNAC4, localization of OsNAC4 was investigated. OsNAC4 was translocated into nucleus during HR cell death and its translocation was regulated by phosphorylation. Next, we compared global gene expressions in OsNAC4 RNAi line using oligonucleotide microarray. OsHSP90 were identified as upregulated gene by OsNAC4. When OsHSP90 was overexpressed in rice cells, loss of plasma membrane integrity was observed.