Host: The Japanese Society of Plant Physiologists
Pages 0193
Molecular mechanisms of plant cell death after the interaction between a disease resistance gene (R) and an avirulence gene are little known. We produced rice cell lines expressing chimeric genes (CRXa), which consist of the extracellular region of CEBiP, a rice receptor gene for chitin elicitor (GN7), and the intracellular region of Xa21, an R gene to rice bacterial blight. GN7 treatment resulted in the enhanced cell death and oxidative burst in CRXa callus, but not in callus expressing the truncated forms. Responses to lipopolysaccharide elicitor among callus lines were the same. These results indicated that GN7 signal was converted to cell death signaling through CRXA. We next analyzed the generation of reactive nitrogen species (NO and ONOO). GN7 treatment caused a great increase of both species in CRXa callus. Treatment with NO but not ONOO scavenger suppressed the cell death, suggesting that NO is involved in Xa21-dependent cell death.
