Supplement to Plant and Cell Physiology Vol. 49
Conference information
Host: The Japanese Society of Plant Physiologists
The role of ADP-ribosylation factor 1 (ARF1) in blue light response
Pages 0206
Details
Host: The Japanese Society of Plant Physiologists
Pages 0206
Blue light receptor phototropin (phot) is involved in various light responses, such as phototropism, chloroplast movement, and stomatal opening, in plants. Phot is chromoprotein consisting of the N-terminal LOV domain and the C-terminal kinase domain.
We previously identified a homologue of ADP-ribosylation factor 1 (ARF1) as a novel interaction partner of phototropin. A recent study further showed that phot specifically interacted with the GTP-bound form of ARF1, through the Ser/Thr kinase domain of phot.
To understand the role of ARF1 in the phot response, we identified phot2 mutant(s) that abolished the interaction with ARF1(GTP). Furthermore, we transformed such mutant phot2-GFP driven by the phot2 promoter in the Arabidopsis phot1phot2 mutant and analyzed the physiological response of the plant and the intracellular localization of mutant phot2:GFP.
In this session, we show the results and discuss the molecular mechanism of blue light signal transduction.
