Abstract
phot1 and phot2 act as blue light receptors for phototropism, chloroplast relocation, stomatal opening and leaf expansion. In the blue light-induced stomatal opening, blue light signal perceived by phot1 and phot2 induces activation of the plasma membrane H+-ATPase and stomatal opening. However, signaling pathway between phots and H+-ATPase is largely unknown. In this study, we performed the screening of mutants, which show opened stomata phenotype, from EMS-treated phot1 phot2 double mutant in Arabidopsis. One of the isolated mutants, named db10-2, showed always opened stomata phenotype, but this mutant was sensitive to abscisic acid. Interestingly, the H+-ATPase in guard cells of db10-2 was always activated, therefore, this mutant may show opened stomata phenotype. Now, we are trying to identify a mutation gene in db10-2 by map-based cloning.
