Host: The Japanese Society of Plant Physiologists
Pages 0262
The flo2 mutant shows decreased expression of most genes involved in starch synthesis and storage proteins. Flo2 gene may encode a multi-functional regulatory factor for biosynthesis of storage substances in the rice grain. In order to isolate the Flo2 gene, we generated the F2 lines crossed between the flo2 mutant and Kasalath. Using the recessive F2 lines, we mapped the Flo2 locus in detail and determined to be in the 37 kb region. The nucleotide sequence analysis of the corresponding region revealed a point mutation in an ORF, which generated a translation stop codon. The corresponding mutation was found in all F2 lines showing the flo2 phenotype. We also analyzed the corresponding ORF in other flo2 mutants which were independently established, and detected that similar mutations occurred in this ORF on these mutants. These results strongly suggest that this ORF is the Flo2 gene.
