Host: The Japanese Society of Plant Physiologists
Pages 0273
Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans widely distributed in plants. AGPs are implicated in several aspects of cell wall biology including the signaling of cell context and cell expansion, yet remain elusive in terms of their mechanistic involvement in cell processes. They are characterized by large amounts of carbohydrate components rich in galactose and arabinose, and protein components rich in hydroxyproline. The study of glycoside hydrolases that cleave AGPs is very important because the enzymes specific to particular sugar residues and type of glycosidic linkage will provide useful tools for the structural analysis of the sugar moieties of AGPs. Recently, we demonstrated that Streptomyces avermitilis possess the both exo-1,3-galactanase and endo-1,6-galactanase, suggesting S. avermitilis possess more AGPs degrading enzymes. By using the information of genome sequence of S. avermitilis, we cloned some possible AGPs degrading enzymes. We will present how these enzymes act on AGPs.
