Host: The Japanese Society of Plant Physiologists
Pages 0290
We have been utilizing LC-ESI-MS/MS for high-sensitive plant hormone analysis. Our goal is to establish a method for comprehensive analysis of all plant hormones and their precursors and metabolites. As reported in this meeting last year, we have established a method for bioactive hormones (GA1, GA4, ABA, IAA, JA, SA, cytokinins, brassinosteroids). To better understand how hormone levels are regulated in plants, the hormone precursors and metabolites should also be analyzed. We have previously reported the analysis methods for 14 GA related compounds and 7 ABA related compounds. In this presentation, we will report the analysis method for JA related compounds. Although JA plays important roles in various stress responses, the biosynthesis and metabolism pathways have not been fully clarified yet. The analytical method for OPDA, OPC:8, JA-leucine, JA-isoleucine will be discussed.
