Host: The Japanese Society of Plant Physiologists
Pages 0296
Some plants grew better in spite of low inorganic nitrogen concentration under the condition of organic matter application as nitrogen sources, compared with the chemical fertilizer application. This suggested the direct uptake of organic nitrogen by such a plants. PEON is considered to be the most promising nitrogen sources of organic nitrogen in soil. In this study, we tried to show the evidence of direct uptake of PEON using the immunohistochemical method.
We collected PEON from a soil and purified by dialysis. As nitrogen source, PEON was applied to sand medium on which chingensai, lettuce and syungiku were grown for 14 days. The growth of three differed seedlings applied with PEON were much better than those applied with NH4NO3. Roots of lettuce grown on sand-medium applied with PEON were subjected to immunohistochemical staining with anti-PEON antibody and Hrp-DAB system . Then, positive reactions with PEON in lettuce roots were obtained
