Abstract
Ascorbate (AsA) content in suspension cells of peach (Prunus persica cv. 'Yuuzora') derived from fruit tissue was studied. At day 2 after transfer to new medium, a highest level of 0.4 μM/gFw was observed. AsA content was gradually decreased with the increase in total cell volume, reaching approximately 0.13 μM/gFw at day 18. The ratio of reduced form was 80-90 % which then decreased after day 10. No increase in total cell volume was observed after day 18. The pH of the medium varied lot by lot, although there was a tendency that it was around 4 by day 7, increasing after that, above 5 after day 18. The change in the levels of mRNAs encoding enzymes involving in AsA biosynthesis (L-galactonolactone dehydrogenase, L-galactose dehydrogenase, GDP-mannose pyrophophorylase, GDP-mannose epimerase, aldo-keto reductase) will be presented.
