Abstract
Suaeda japonica is one of highly salt-tolerant halophyte, from which we prepared cDNA library followed by the functional screening for salt-tolerant related genes using E.coli established in our laboratory. We could get the s284 cDNA by the screening, which encoded a highly hydrophilic protein consisting of 670 amino acid residues, including the repetitive amino acid sequence motif, PSTTK and its sisters. s284 encoded the protein having to the similarity to TNP1 of the transposase of Antirrhinum majus. Southern analysis using the nucleotide sequence for the PSTTK repeats as a probe revealed that the similar nucleotide might present in the halophyte Suaeda maritime, but not non-halophyte plants. We could get the genomic lambda DNA clone harboring the En/Spm transposable element, Tsj1, which encoded s284 sequence as exons.
